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Effect of resveratrol on LPS-induced AP-1 activation. N9 cells (A) or murine primary astrocytes (B), pretreated with different concentrations of resveratrol (RV) for 30 min, were stimulated with 0.5 μg/mL LPS for 4 h. DNA-binding activity of AP-1 in the nuclear extract was examined by EMSA using a biotin-labeled AP-1 consensus probe. A 200-fold molar excess of cold probe was used to confirm the specificity. Data are representative of 3 separate experiments with similar results.
