Table 1.
Description of viruses used in the study
| Virus | Capsid modification | References | |
|---|---|---|---|
| Replication deficient virusesa | AdTL | Wild type 5 capsid | |
| DATL | -Y477A substitution in DE loop of fiber knob for CAR ablation | ||
| -Penton base's RGD domain mutated to RGE for αvβ integrin ablation | [41] | ||
| -6xhistidine carboxy-terminal tag for the propagation in 293.HissFv.rec cells | |||
| AdTLG | -Fiber shaft's KKTK domain mutated to GATK for HSPG ablation | [27] | |
| AdTLGR | -RGD insertion in HI loop of fiber knob for αvβ integrin targeting | ||
| -Fiber shaft's KKTK domain mutated to GATK for HSPG ablation | [27] | ||
| AdTLYG | -Y477A substitution in DE loop of fiber knob for CAR ablation | ||
| -Fiber shaft's KKTK domain mutated to GATK for HSPG ablation | [21,27] | ||
| AdTLYGR | -Y477A substitution in DE loop of fiber knob for CAR ablation | ||
| -RGD insertion in HI loop of fiber knob for αvβ integrin targeting | |||
| -Fiber shaft's KKTK domain mutated to GATK for HSPG ablation | [21,27] | ||
| AdTLY | -Y477A substitution in DE loop of fiber knob for CAR ablation | [21] | |
| Ad5luc1RGD | -RGD insertion in HI loop of fiber knob for αvβ integrin targeting | [42] | |
| AdTLRGDK | -Fiber shaft's KKTK domain mutated to RGDK for αvβ integrin targeting | [30] | |
| -HSPG ablation via mutated KKTK | |||
| Replicating viruses | WT | -Replicating wild type 5 virus | |
| WT-RGD | -RGD insertion in HI loop of fiber knob for αvβ integrin targeting | [43] | |
| WT-RGDK | -Fiber shaft's KKTK domain mutated to RGDK for αvβ integrin targeting | [30] | |
| -HSPG ablation via mutated KKTK | |||
aAll replication deficient viruses are deleted for E1A and have both luciferase (Luc) and green fluorescent protein (GFP) as marker genes.
CAR, coxsackie virus and adenovirus receptor; HSPG, heparan sulphate proteoglycan; VP, viral particles; pfu, plaque forming unit.