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. 2010 Aug 26;8:104. doi: 10.1186/1477-7827-8-104

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Copyright ©2010 Pinto et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Time- and concentration-dependent effects of phosphoramidon on human sperm motility. Motility analysis was performed manually (A, B) or using a computer-assisted sperm analysis (CASA) system (C). (A) Effects of phosphoramidon (1 nM-1 μM) or its solvent on progressive motility (grade a+b sperm) at different times of incubation (B) Effects of phosphoramidon (1 μM) on progressive motility (grade a+b sperm), non-progressive motility (grade c sperm) and immotility (grade d sperm) at different times of incubation. (C) Effects of phosphoramidon (1 μM) on grade a and grade b sperm at different times of incubation. Bars are means with SEM of 6-13 different experiments and represent percentage changes in motility in samples treated with phosphoramidon relative to the value observed at the same time in untreated (A) or solvent-treated (B, C) paired controls. *P < 0.05, significant difference vs. control responses.