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Primers, restriction enzymes and length of digested fragments of IL-10 promoter polymorphism
| Polymorphism | Primer sequence | Annealing temperature | Restriction enzyme | Allele size |
|---|---|---|---|---|
| IL-10-1082G/A | 5'-CTCGCTGCA ACCCAACTGGC-3' | 58°C | MnlⅠ | A:139 bp |
| 5'-TCTTACCTATCCCTACTTCC-3' | G:106,33 bp | |||
| IL-10-819C/T | 5'-TCATTCTATGTGCTGGAGATGG-3' | 59°C | Mae III | C:125,84 bp |
| 5'-TGGGGGAAGTGGGTAAGAGT-3' | T:209 bp | |||
| IL-10-592C/A | 5'-GTGAGCACTACCTGACTAGC-3' | 58°C | RsaI | C:412 bp |
| 5'-CCTAGGTCACAGTGACGTGG-3' | A:175,237 bp |
