Table 1.
List of genes and primer and probe nucleotide sequences.
| Gene | Primer# | Reference | Primer (5'-3') |
|---|---|---|---|
| OC | F | NM_199173.3 | GAAGCCCAGCGGTGC |
| R | CACTACCTCGCTGCCCTCC | ||
| FAM | TGGACACAAAGGCTGCACCTTTGCT | ||
| OPG | F | NM_002546.3 | GCAGCGGCACATTGGAC |
| R | CCCGGTAAGCTTTCCATCAA | ||
| FAM | TGCTAACCTCACCTTCGAGCAGCTTCGTA | ||
| RANKL | F | NM_003701.2 | CGTTGGATCACAGCACATCAG |
| R | NM_033012.2 | GCTCCTCTTGGCCAGATCTAAC | |
| FAM | CAGAGAAAGCGATGGTGGATGGCTCAT | ||
| BMP2 | F | NM_001200.2 | AACACTGTGCGCAGCTTCC |
| R | CTCCGGGTTGTTTTCCCAC | ||
| FAM | CCATGAAGAATCTTTGGAAGAACTACCAGAAACTG | ||
| TGF-β1 | F | NM_000660.3 | CGAGCCTGAGGCCGACTAC |
| R | AGATTTCGTTGTGGGTTTCCA | ||
| FAM | - | ||
| SPP1 | F | NM_001040060.1 | CTCAGGCCAGTTGCAGCC |
| R | NM_001040058.1 | CAAAAGCAAATCACTGCAATTCTC | |
| FAM | NM_000582.2 | AAACGCCGACCAAGGAAAACTCACTACC | |
| SPARC | F | NM_003118.2 | ATCTTCCCTGTACACTGGCAGTTC |
| R | CTCGGTGTGGGAGAGGTACC | ||
| FAM | CAGCTGGACCAGCACCCCATTGAC | ||
| IBSP | F | NM_004967.2 | TGCCTTGAGCCTGCTTCC |
| R | GCAAAATTAAAGCAGTCTTCATTTTG | ||
| FAM | CTCCAGGACTGCCAGAGGAAGCAATCA | ||
| MMP-1 | F | NM_002421 | CTCAATTTCACTTCTGTTTTCTG |
| R | CATCTCTGTCGGCAAATTCGT | ||
| FAM | CACAACTGCCAAATGGGCTTGAAGC | ||
| MMP-3 | F | NM_002422 | TTTTGGCCATCTCTTCCTTCA |
| R | TGTGGATGCCTCTTGGGTATC | ||
| FAM | AACTTCATATGCGGCATCCACGCC | ||
# Primer = Forward (F), Reverse (R), FAM-labeled TaqMan® probe (FAM). All assays are TaqMan® assays except for TGF- β1 (SYBRGreen assay).