Table 3.

Serological and PCR results for eight patients infected with Bartonella koehlerae.

Patient	Date	IFA Reciprocal Titers				PCR Results
		B. koehlerae	B. henselae	Bvb II	R. rickettsii	ITS PCR	rpoB PCR
	10-2008	64	<16	<16	<16	Bvb*,a	Neg
1	02-2009**	<16	<16	<16	<16	B. koehleraea	B.koehleraea
1	04-2009**	<16	<16	<16	<16	Bsppb	Neg
2	03-2009	<16	<16	32	64	B. koehleraec	B.koehleraea
2	04-2009	NA	NA	NA	NA	Neg	Neg
2	07-2009**	<16	<16	<16	16	B. koehleraea	Neg
2	10-2009**	<16	<16	<16	<16	Neg	Neg
3	03-2009	64	<16	64	<16	B. koehleraea	B.koehleraea
3	04-2009	NA	NA	NA	NA	B. koehleraec	Neg
3	05-2009**	64	<16	32	<16	Neg	Bvb*,a
3	08-2009**	256	<16	16	<16	Neg	Neg
3	09-2009**	<16	<16	<16	<16	Neg	Neg
4	4-13-2009	<16	<16	<16	32	B. koehleraeb	Neg
4	4-30-2009**	<16	<16	<16	<16	Neg	Bvb*,a
4	6-02-2009**	<16	<16	32	<16	Neg	Neg
4	6-09-2009**	<16	<16	64	16	Neg	Neg
4	6-30-2009**	64	<16	64	<16	Neg	Neg
5	05-2009	NA	NA	NA	NA	B. koehleraea	B.koehleraea
6	05-2009	NA	NA	NA	NA	B. koehleraea	B.koehleraea
7	04-2009	64	<16	32	128	B. koehleraeb	Neg
7	06-2009	64	<16	64	64	Neg	Bvb*,a
7	07-2009	64	<16	<16	64	B. henselaea	Neg
7	11-2009**	256	<16	<16	16	B. henselaea	Neg
8	04-2009	64	<16	<16	16	B. koehleraeb	Neg
8	05-2009	64	<16	<16	<16	B. koehleraea	B.koehleraea
8	07-2009	64	<16	<16	32	Neg	Neg
8	08-2009	<16	<16	32	<16	Neg	Neg

All ITS and rpoB PCR amplicons were sequenced to confirm genus and species identity.

*Bvb = Bartonella vinsonii subsp. berkhoffii genotype II

Bspp = amplicon generated with 16S-23 S ITS primers, but unable to obtain a DNA sequence.

** Denotes post-antibiotic testing. NA = Sample not available.

^a = amplicon sequenced from blood or serum

^b = amplicon sequenced from BAPGM enrichment blood culture

^c = amplicons sequenced from both blood and BAPGM enrichment blood culture