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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Mol Microbiol. 2010 Apr 27;76(5):1306–1321. doi: 10.1111/j.1365-2958.2010.07174.x

Table 4.

Bacterial strains and plasmids used in this study

Strain/plasmid Genotype/description Reference
Strain
86-24 Wild-type EHEC strain (serotype O157:H7) (Griffin et al., 1988)
BL-21(DE3) F– ompT hsdSB(rB–, mB–) gal dcm (DE3) Invitrogen
VS145 86-24 qseA mutant (Sperandio et al., 2002a)
VS151 VS145 with plasmid pVS150 (qseA complement) (Sperandio et al., 2002a)
MK11 EHEC strain 86-24 with plasmid pMK40 This study
MK14 EHEC strain 86-24 with plasmid pVS23 This study
MK15 VS145 with plasmid pVS23 This study
Plasmid
pET28 Cloning/ expression vector Novagen
pMK08 EHEC 86-24 qseA in pET28 This study
pVS23 Regulatory region of LEE1 in pBluescript (Sperandio et al., 2002a)
pRS551 lacZ reporter gene fusion vector (Simons et al., 1987)
pVS150 EHEC 86-24 qseA in pACYC177 (Sperandio et al., 2002a)
pMK40 QseA promoter sequence in pRS551 This study