Table 5.
Primers used in this study.
| Primers for qPCR and RNA slot blots |
|
|---|---|
| escU_RT_F1 | TCCACTTTGTATCTCGGAATGAAG |
| escU_RT_R1 | CAAGGATACTGATGGTAACCCTGAA |
| escC_RT_F1 | GCGTAAACTGGTCCGGTACGT |
| escC_RT_R1 | TGCGGGTAGAGCTTTAAAGGCAAT |
| escV_RT_F1 | TCGCCCCGTCCATTGA |
| escV_RT_R1 | CGCTCCCGAGTGCAAAA |
| grlA_RT_F1 | CCGGTTGTTCCAGGACTTTC |
| grlA_RT_R1 | TAAGCGCCTTGAGATTTTCATTT |
| espA_RT_F1 | TCAGAATCGCAGCCTGAAAA |
| espA_RT_R1 | CGAAGGATGAGGTGGTTAAGCT |
| eae_RT_F1 | GCTGGCCCTTGGTTTGATCA |
| eae_RT_R1 | GCGGAGATGACTTCAGCACTT |
| espX3′ (Z5212)_RT_F1 |
AACCACGCAGTTCCCCATAA |
| espX3′ (Z5212)_RT_R1 |
GTTAGACAATTTAGAAAAACGATTGAGATG |
| espX3′ (Z5213)_RT_F1 |
GGGACAAATTTTAGCGGTTCTACA |
| espX3′ (Z5213)_RT_R1 |
CGTCCACTTTGTTGGTGTCTTAAT |
| espY5′ (Z5214)_RT_F1 |
CGTCGTACTAAAGCGCCATTT |
| espY5′ (Z5214)_RT_R1 |
ACTGAGGACAAAGTTAAGAGATTTGAGA |
| ygeK_RT_F1 | TTTTCCATACGCATCCTTTCG |
| ygeK_RT_R1 | TTTGTAGCAAAGATGCCGTATATTG |
| yqe1_RT_F1 | GGCCTGAGCACCGAAAATTA |
| yqe1_RT_R1 | CTGGCTGGCGTTGTCAGA |
| Z0326_RT_F1 | GCAGGCTGTTCTGACACCAA |
| Z0326_RT_R1 | CCAAATGATGGCATATTCCTTTTT |
| Z2967_RT_F1 | CAAGACACTGTAATTGAACCTCATCA |
| Z2967_RT_R1 | CCGTCGCAATGCCTTTTAAA |
| Z2968_RT_F1 | CGGATTAATTCTGAAGCACGAA |
| Z2968_RT_R1 | TCGGCATCAAGCCACATAGA |
| Z2969_RT_F1 | CCTTTTCACTGATCGCACCTTT |
| Z2969_RT_R1 | TCGAGCAGCTTCGTTAGATGTG |
| Z2971_RT_F1 | GCCCACGCAATCCGTTT |
| Z2971_RT_R1 | GATTGTCGCCGCAGTTCAA |
| Z2972_RT_F1 | TTGATTTTCGCGGTGAACTG |
| Z2972_RT_R1 | TTTTTTCGGATGGAACAATTCC |
| Z2973_RT_F1 | GTGCAGCCCGTGACTCATT |
| Z2973_RT_R1 | TCAGCGTAAAAAGCAACGTCAT |
| Z2974_RT_F1 | CAGGGCAGCCCAGAAAGATA |
| Z2974_RT_R1 | GTGGCTATTGCGATGGTCAGT |
| Z2975_RT_F1 | CCTGGCGAACCGGAAAC |
| Z2975_RT_R1 | AACGTTATCGATGCGTTGCA |
| Z2976_RT_F1 | CGTCGTCACATCCAGACTGAA |
| Z2976_RT_R1 | TGCGCGACGCGGAT |
| Z2977_RT_F1 | CCTCTGCAATCGTCGATGTG |
| Z2977_RT_R1 | TCGGCATGGCGATGTGT |
| Z4181_RT_F1 | GAGCGGGTCAACGTCTCAA |
| Z4181_RT_R1 | GAGGTTTCATTGGTGGGAACA |
| Z4185_RT_F1 | TGGCAATCATCAAACGGCTAT |
| Z4185_RT_R1 | GAGAGCGCCATCAGGAAATTC |
| Z4189_RT_F1 | ACCAACTCCGGTTCAGAATATTTT |
| Z4189_RT_R1 | CGTCGGTTGTTAATTTTGTTGAGA |
| qseA _RT_F1 | CGCAAGGGAGATTTGTGACTAAT |
| qseA_RT_R1 | GGCACCCGCCGTTAGC |
| rpoA_RT_F1 | GCGCTCATCTTCTTCCGAAT |
| rpoA_RT_R1 | CGCGGTCGTGGTTATCTG |
| ler_dotblot_F1 | GAGATTATTTATTATGAATA |
| ler_dotblot_R1 | TCTTCTTCAGTGTCCTTCAC |
| dsrA_F2 | GCACAATAAAAAAATCCCGA |
| dsrA_R2 | ATTGCGGATAAGGTGATGAAC |
| Primers for RT-PCR | |
| Z2966-69_F1 | TTAATTGCCCATCAGGAAGC |
| Z2966-69_R1 | TTCCTGATGCTGCGTATTTG |
| Z2970-73_F1 | TCAATGTGAATGCCCCCTAT |
| Z2970-73_ R1 | ATGCGATATTCCCCAGCTTA |
| Z5212-Z5213_F1 | GGCACACCACCAAACCACGC |
| Z5212-Z5213_R1 | GCTCTTGCCTTACACTATA |
| Primers for EMSAs/ footprinting |
|
| QseA_pETF1 | CATATGGAACGACTAAAACGCATGTCGGTG |
| QseA_pET28R1 | AAGCTTTTACTTCTCTTTCCCGCGCCCGTG |
| LerProximal_F1 Ler promoter -173 F |
CGGGATCCCGATGATTTTCTTCTATATCATTG |
| LerProximal_R1 Ler promoter -42 R |
CGGAATTCCGCGACCTTATCAGGAAGGACC |
| LerDistal_F2 | CGGAATTCCTGGGGATTCACTCGCTTGC |
| LerDistal_R1 | TCTAATGTGTAAAATACAT |
| QseA_EMSA_F1 | TAGATACCTGTTGGCACAAGTACCCGGCACAC |
| QseA_EMSA_R1 | CATTATTCACTCTGACTTAAAAGTGATTTAGA |
| EspX3′ (Z5212)_EMSA_F1 |
TCCTCCGGCGTGTAATATTG |
| EspX3′ (Z5212)_EMSA_R1 |
TATCCATAAATCACCTTGAT |
| Z4189_EMSA_F1 | GCATCATTACAAACATTGAC |
| Z4189_EMSA_R1 | TGAAACAGATGACTTTGATA |
| Z2969_EMSA_F1 | GTCGCTCATGGCAACTTGAC |
| Z2969_EMSA_R1 | AGCTGTTAGCCAAAGCGTCT |
| coxT_EMSA_F1 | CAATCTTTCCCTTTCCTCAC |
| coxT_EMSA_R1 | TGATGGTTATACCTGACATC |
| kan_EMSA_F1 | CCGGAATTGCCAGCTGGGGCG |
| kan_EMSA_R1 | TCTTGTTCAATCATGCGAAACGATCC |
| amp_EMSA_F1 | GGAATTCGAAAGGGCCTCGTGATACGC |
| amp_EMSA_R1 | CGGGATCCGGTGAGCAAAAACAGGAAGG |
| Primers for primer extension |
|
| qseA_PE_F1 | CACGATAACGGGAAACAGACTCATGTTGACCT |
| qseA_PE_R1 | TGACACCGTCTGACTGATGGACGA |
| K983 | GGACTTGTTGTATGTG |