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. 2010 Jul 21;21(9):1093–1103. doi: 10.1089/hum.2010.040

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Copyright 2010, Mary Ann Liebert, Inc.

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FIG. 5. — High neuronal transduction numbers in targeted regions. (A) Cellular counting of randomized adjacent, histology-processed sections stained against neuronal marker (anti-NeuN) and HA tag epitope (anti-HA). (B) High neuronal transduction levels in the brainstem (82 ± 7.8%, n = 4) and in the thalamus (68 ± 11.3%, n = 7). No significant difference was found between levels of transduction efficiency in these two regions (p > 0.05). Microscopic analysis was performed on NHP tissue perfused 5 weeks after AAV2-hASM-HA infusion. Scale bars: for whole brain mounts, 2 cm; for immunostained sections, 500 μm.