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Efficient neuronal transduction in brainstem. Immunostained brainstem sections indicate neuron-specific transduction (A–C) with no transduction observed in astrocytes (D–F) and/or microglia (G–I) (see high-magnification panels on the right). Note that the total number of colocalized NeuN/HA-positive cells was 61 of 63, representing 97% neuronal specificity. Scale bar: (A–I) 500 μm. Color images available online at www.liebertonline.com/hum.
