Figure 10. The OsAP2-39 transcription factor directly activates OsNCED-1 and EUI and its activity is affected by the environmental hormonal conditions.

Transcription activation of OsAP2-39 using GUS based transient assay and 4-methylumbelliferyl β-D-glucuronide (MUG) as the substrate. (A) Illustration of the reporters, the effector and the loading calibrator constructs (not drawn to scale). Tobacco leaves were bombarded with the construct containing the OsNCED-1 (B) or the EUI promoter (C) fused to the GUS gene and then leaves were incubated on MS media supplemented with water (0), 10 µM ABA or 100 µM GA. The 4-methyl umbelliferone (MU) was used to generate the standard curve. Bars represent mean ±SE. (n = 8).