Figure 6. Rescued lines present differential response to shade in developmental time traits and circadian rhythms.

Shade avoidance response phenotypes of elf3-1 rescued lines. Mutant elf3-1 plants with a stable insertion of CCR2::luc were transformed with the ELF3-Bay or ELF3-Sha genes driven by their own promoters. (A) T1 transformants were grown in 12∶12 under simulated sun or shade and measured for their shade avoidance response. Each data point represents the average ± standard error for each genotype and condition, and numbers beside each point represent the number of plants measured. Shaded versus non-shaded areas indicate measurements from plants in simulated shade and sun respectively. Bar plots show the increment ± standard error of each trait in simulated sun with respect to simulated shade. Asterisks on each bar plot indicate significant differences. ** - ANOVA test p<0.01. (B) and (C)- Luminescence rhythms of T1 transformants were monitored in 5 experiments under constant red light and 5 experiments under constant red plus far red light. (B) Circadian rhythms represent the average luminiscence ± standard error of 60–150 plants with a Relative Amplitude Error below 0.5. One representative experiment in each light condition is shown. Shaded regions correspond to subjective nights. (C) Line plots represent the average period or amplitude ± standard error for all 10 experiments performed. Numbers beside each line end represent the number of plants measured. Shaded versus non-shaded areas indicate measurements from plants in simulated shade and sun respectively. Bar plots represent the difference in period and amplitude between cR+FR and cR per genotype. ** - ANOVA test p<0.001.