Table 2. Presence (+) or absence (−) of C. elegans homologous proteins of the DEG-3 subfamily members in various species.
| Isolates | ACR-23 | MPTL-1 | ACR-20 | DES-2 | DEG-3 | ACR-5 | AAD-1566 EC50 in µM |
| C. elegans | + | − | + | + | + | + | 0.19±0.05 |
| C. japonica | + | − | + | + | + | + | <0.1 |
| C. briggsae | + | − | + | + | + | + | 0.90±0.06 |
| C. remanei | + | − | + | + | + | + | 1.25±0.29 |
| C. brenneri | +a | − | + | +a | +a | + | 0.38±0.08 |
| P. pacificus | − | − | − | + | + | − | 43±28 |
| C. elegans cb27 | − | − | + | + | + | + | 25±16 |
| H. contortus b | − | + | − | + | + | + | 0.003 |
| H. bacteriophora | − | + | − | + | + | + | n. d. |
| B. malayi b | − | − | − | + | + | − | n. d. |
| S. ratti b | −* | −* | −* | −* | + | − | >250 |
| M. incognita b | − | − | − | − | + | + | n. d. |
| M. hapla b | − | − | − | − | + | − | n. d. |
| T. spiralis b | − | − | − | − | −* | −* | n. d. |
| Vertebrates | − | − | − | − | − | − | n. d. |
The AAD-1566 EC50 values with standard errors of the mean was calculated using sigmoidal dose-response (variable slope) curves from in vitro data points with four replicates. Haemonchus contortus EC50 was determined in a previous study [10].
a
Multiple copies of the gene are found in the genome.
b
Obligatory parasites.
c
See Table 1 for a list of vertebrate species analysed.
*: S. ratti has a more remote relative of MPTL-1/DES-2 kind, much as T. spiralis of DEG-3/ACR-5.