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. 2010 Sep 9;6(9):e1001092. doi: 10.1371/journal.ppat.1001092

Figure 2. The beta- and gamma-herpesvirus CHPKs phosphorylate Rb.

Figure 2

(A) Lysates from Saos-2 cells transfected with plasmids encoding Rb and either an empty vector (EV) or one encoding the indicated human herpesvirus (1–8) CHPKs were harvested 48 hpt and analyzed by Western blot with the indicated antibodies. Rb, total Rb; 807, Rb phosphorylated on serine residues 807 and 811; 780, Rb phosphorylated on serine 780; 821, Rb phosphorylated on threonine 821; HA, epitope tag; Tubulin, tubulin loading control. (B) Both wild type and kinase-deficient (KD) alleles of the beta- and gamma-herpesvirus CHPKs (4–8) were analyzed in the Saos-2 Rb phosphorylation assay as described above. (C) Lower levels of expression plasmids for the HHV-4 and HHV-7 CHPKs were analyzed in the Saos-2 Rb phosphorylation assay as described above. Please see the Materials and Methods section for details.