Table 2. CHPK alleles used in this study.
HHV number | Common name | Virus strain | CHPK name | Predicted MW | Accession number | KD mutation |
1 | HSV-1 | KOS | UL13 | 57 kDa | HM584913 | N/A |
2 | HSV-2 | 186 | UL13 | 57 kDa | NC_001798 | N/A |
3 | VZV | VZV32 | ORF47 | 57 kDa | AF314218.1 | N/A |
4 | EBV | B95-8 | BGLF4 | 48 kDa | DQ279927 | K102I |
5 | HCMV | AD169 | UL97 | 78 kDa | X17403 | K355M |
6 | HHV-6 | U1102 | U69 | 64 kDa | X83413.1 | K218G |
7 | HHV-7 | RK | U69 | 63 kDa | NC_001716 | K202N |
8 | KSHV | GK18 | ORF36 | 50 kDa | AF148805 | K108Q |
The indicated viral strains (delineated by the human herpesvirus number 1–8 as well as the commonly-used virus name) served as templates for PCR reactions generating the CHPK alleles analyzed in this study. Accession numbers indicate the GenBank entries used for sequence confirmation. The amino acid substitutions in the kinase-deficient (KD) mutant alleles are indicated.