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. 2010 Aug 23;107(36):15874–15879. doi: 10.1073/pnas.1006901107

Fig. 1.

Fig. 1.

MLV-related gag gene sequences detected in blood DNA from CFS patients. (A) Results of PBMC DNA from CFS patient samples 1–25 (of 41 samples examined) amplified after the first round of nested PCR using a previously published primer set (419F/1154R): targeting gag gene. (B) Results of PBMC DNA from the 25 CFS samples after completing the second round of nested PCR using an in-house–designed PCR primer set (NP116/NP117). (C) MLV-related gag gene RNA sequences are detected in plasma of CFS samples by RT-nested PCR. Results of RT-nested PCR for RNA derived from the plasma samples of CFS patients 4–17 are shown. The positions of expected sizes of the “positive” PCR amplicons are indicated by arrows. M, DNA ladder size markers. All positive PCR amplicons with the expected size have been confirmed by DNA sequencing.