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. 2010 Aug 23;107(36):15874–15879. doi: 10.1073/pnas.1006901107

Fig. 6.

Fig. 6.

Testing of CFS patients’ and healthy blood donors’ samples positive for MLV-like gag gene sequences for the presence of mouse DNA contamination using the semi-nested PCR assay targeting mouse-specific mtDNA. Serial dilutions of mouse DNA were spiked into 35 ng of human DNA and used as the controls of the assay sensitivity. The first round of mouse mtDNA semi-nested PCR (A) detected 10 fg of mouse DNA, and the second round of the semi-nested PCR (B) detected 2.5 fg of mouse DNA in the presence of 35 ng human background DNA. No evidence of mouse DNA contamination could be found by either round of mouse mtDNA semi-nested PCR in the PBMC DNA (35 ng) of CFS patients (patients 8, 17, 20, and 25); three blood donors (BD-22, BD-26, BD-28) tested positive and two blood donors (BD-21 and BD-23) tested negative for the MLV-like virus gag gene sequences. Healthy blood donors’ samples positive for MLV-like gag gene sequences are labeled by asterisks. Lane 0: 35 ng of human DNA without spiking any mouse DNA. Lane H2O: No DNA template. M: 100-bp DNA ladder mix.