Fig. 3.
Reversed-phase high-performance liquid chromatography (RP-HPLC) of the small-molecule reactants and products following incubation of 
with excess Ar-NHOH and O2. 
(15 μM) was incubated with 450 μM Ar-NHOH and ∼0.9 mM O2 for 20 min at 0 °C. Small molecules were separated from the enzyme and analyzed as described in SI Text (blue). A control experiment was carried out under identical conditions, except for omission of 
(green). A solution containing 400 μM each of Ar-NH2, Ar-NHOH, and Ar-NO2 was also analyzed (red).