Fig. 2.

Molecular characterization of the SUPPRESSOR OF VARICOSE. (A) The mapping interval that contained SOV included nine genes (depicted by broad arrows) and was defined by recombination break points indicated by the heavy vertical black arrows. (B) The L.er genomic DNA used for complementation analysis is represented by the dark gray bars, which were positioned to correspond to the region shown above the bars in A. Each rescue construct contained ≈2-kb upstream sequences, 1-kb downstream sequences, and all introns and exons. Note that the 5-kb scale bar works for both A and B. (C) Semiquantitative RT-PCR analysis of candidate gene expression in 7-d seedlings. Three of the candidate genes showed different levels of expression in the two accessions. Note that for At1g77690, reactions with different cycle numbers are shown to confirm differential expression. (D) Cartoon representation of the protein polymorphisms found in three SOV candidate genes. (Scale bar: 100 amino acids.)