Fig. 4.

SOV encodes a conserved protein that localizes to the cytoplasm. (A) A phylogenetic tree, built using amino acid sequences of RNA binding domains (RNB) from SOV and SOV-like proteins. This analysis revealed that SOV as belonging to a conserved cohort that was distinct from RRP44 proteins. To the right, the domain organization of each analyzed protein is depicted. The RRP44 cohort all contained the typical RRP44 domains, whereas those of the SOV cohort all lacked the PIN domain and contained fewer CSD domains. Bootstrap values are indicated in blue. At, Arabidopsis; Sc, yeast; Mm, mouse; Ce, Caenorhabditis elegans; Dm, Drosophila; Os, rice; Sm, Selaginella. (B) Confocal image of CFP expression driven from the 35S promoter. CFP alone (Left) localizes throughout the cytoplasm and nucleus, whereas a translational fusion to SOV localizes to discrete cytoplasmic foci (Center) and a translational fusion to AtRRP44 largely localizes to the nucleus (Right). (Scale bars: 1 mm.)