Fig. 5.

SOV functions in cytoplasmic mRNA decay. (A) Steady-state levels of three mRNAs, relative to the actin internal control. (B) RNA decay analysis comparing three mRNAs in five genotypes: wild-type (Col-0), wild-type (L.er), vcs mutants (vcs-7 in Col-0 and vcs-1 in L.er) and transgenic vcs-7 (Col-0) containing SOV^L.er. (C) Relative expression of RRP44 and SOV in various organs of L.er plants. Both genes are expressed in all tissues analyzed; the relative abundance of SOV and AtRRP44 varied among the organs, but SOV expression was always greater than that of AtRRP44. All three analyzes used real-time RT-PCR. Error bars indicate SEM.