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. 2010 Aug 23;107(36):15963–15968. doi: 10.1073/pnas.1008705107

Fig. 3.

Fig. 3.

Dominant negative effect of the Δ65bp mutant in HEK293 cells. (A) Original traces showing the Na+-dependent cell pH recovery for wild-type NBCe1B (WT) and the Δ65bp mutant. AML: amiloride (1 mM). (B) Summary data for the functional analyses. Control (Cont): cells transfected with vector alone.*P < 0.01 vs. Cont. #P < 0.01 vs. NBCe1B. Numbers observed were 11 (Control), 12 (Δ65bp), 16 (Δ65bp + NBCe1B), and 19 (NBCe1B). (C) Cell surface expression of NBCe1B. Blots with the anti-GFP antibody in biotinylated (2 μL per lane) and total fractions (15 μL per lane) were shown. Equal protein loading in the biotinylated fraction was confirmed by blots with the anti-Na/K pump antibody. Control (Cont): cells transfected with vector alone.