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. 2010 Aug 23;107(36):15921–15926. doi: 10.1073/pnas.1010209107

Fig. 1.

Fig. 1.

PD patient-derived DA neurons survive after transplantation into the adult striatum of unlesioned rats. (A) Assembled images of K1, S1, FF 17–5, and FF 21–26 PDiPS cell grafts immunostained for human NCAM (red), TH (green), βIII-tubulin (blue), or Hoechst (H; blue) 4 wk after intrastriatal transplantation of 200,000 cells. A high number of neurons was present in all grafts. (B) Z-stacks of three confocal images of 1 μm thickness show coexpression of TH (green) and hNCAM (red) in engrafted DA neurons derived from all four PDiPS cell lines 4 wk after transplantation. (C and D) Stacked confocal images of DA neurons coexpressing Girk-2 (red) and TH (green) 12 wk after transplantation. (E) Immunostaining for hNCAM (red), GFAP (green), and H (blue) showing moderate astrogliosis around grafts. (F) Immunostaining for human L1 (red), βIII-tubulin (green) and H (blue) showing a high number of neurons at the graft–host interface. (Scale bars: 100 μm in A, E, and F; 20 μm in B–D.)