Fig. 5.
Independent regulation of λ-phage lysis proteins in a bacterial kill switch. Graphs depict the triplicate mean ± SEM. (A) Riboregulator system. (B) Effect of λR-RZ and λS expression on OD600. Addition of glucose was necessary to further reduce PBAD leakage and prevent lysis (see SI Text and Fig. S5 for details). (C) Images illustrating cell lysis were obtained from a movie file (Movie S1) that was recorded at 45 min postinduction. (D) Variable λS expression at constant, medium λR-RZ expression. See Fig. S6 for full dataset. (E) Variable λR-RZ expression at constant, medium λS expression.