Fig. 4.

Further enrichment of zebrafish DCs for functional analyses. (A) Single-cell suspensions were prepared from mpx:eGFP transgenic WKM and labeled with PNA. Myelomonocytes (green gate) were divided into mpx⁻ (black box) and mpx⁺ (blue box). These populations were further subdivided into mpx⁻ PNA⁻ (gray box), mpx⁻ PNA⁺ (red box), and mpx⁺ PNA⁺ (blue box) fractions. (B) After 4 h of culture, mpx⁻ PNA⁻ (gray), mpx⁻ PNA⁺ (red), and mpx⁺ PNA⁺ (blue) fractions were collected and stained with MGG to assess cell morphology. Differentials are presented as mean ± SD, n = 6. (C) mpx⁻ PNA⁺ (red bar), mpx⁺ PNA⁺ (blue bar), and mpx⁻ PNA⁻ (black bar) myelomonocyte fractions were cultured for 16 h with and without LPS. The abundance of il-12p40, csf1r, and iclp1 transcripts were measured by qPCR. Expression is presented as fold induction over nonstimulated controls. Asterisks denote populations with no detectable expression of the transcript. Bars represent mean, n = 3. Error bars represent SD.