Table 1.

Oligonucleotide primers used for detection of different chemokines

Gene	Primer Sequences	Annealing Temp. (°C)
KC/Cxcl1	Forward: 5′-ACCGAAGTCATAGCCACACTC -3′ Reverse: 5′-TGGGGACACCTTTTAGCATC -3′	52
Mip2/Cxcl2	Forward: 5′-CCCAGACAGAAGTCATAGCCAC -3′ Reverse: 5′-GCCTTGCCTTTGTTCAGTATC -3′	52
TNFα	Forward: 5′-AAGGGAGAGTGGTCAGTTGCC -3′ Reverse: 5′-CCTCAGGGAAGAGTCTGGAAAGG -3′	55
Rantes	Forward: 5′-GGAGTATTTCTACACCAGCAGC -3′ Reverse: 5′-TCTTGAACCCACTTCTTCTCTG -3′	52
Cxcl9	Forward: 5′-GAGCTAGATAGACCTCACCAAG -3′ Reverse: 5′-CCATTAGCACCATCTCTGA -3′	55
Cxcl10	Forward: 5′-TCGCTCAAGTGGCTGGGATG -3′ Reverse: 5′-TAGGGAGGACAAGGAGGGTGTG -3′	55
Gapdh	Forward: 5′-TGGTTTGACAATGAATACGGCTAC -3′ Reverse: 5′-GGTGGGTGGTCCAAGGTTTC -3′	55
Rotavirus VP6	Forward: 5′-GCGGTAGCGGTGTTATTTCC -3′ Reverse: 5′-TTGTTTTGCTTGCGTCGG -3′	55
Rotavirus NSP3	Forward: 5′-TGTCAAGAGAATACCTGGGAAATC -3′ Reverse: 5′-GGAATCATCAACTTCAACTTCACC -3′	54
Ccl12	Forward: 5′-CACCATCAGTCCTCAGGTAT -3′ Reverse: 5′-GGACGTGAATCTTCTGCTTA -3′	54

Temp = temperature