Fig. 8.

Sphingolipid levels in lipoprotein particles. Blood was collected after 12 h of fasting from healthy normolipemic subjects and equal amount (100 µg) of VLDL, LDL, and HDL subclasses (HDL₂ and HDL₃) was extracted and analyzed using HPLC/MS/MS as described in “Methods.” A: Levels of molecular species of SM. B: Levels of molecular species of Cer. C: Levels of Sph, dhSph, S1P, and dhS1P. Levels of sphingolipid species were calculated per nmol lipoprotein as described in “Methods.” n = 3 pooled plasma samples, except for Cer, Sph, dhSph, S1P, and dhS1P in HDL₂ and HDL₃, n = 8 pooled plasma samples. Data are expressed as mean ± SE.