TABLE 4.

Effect of type of anticoagulant on sphingolipid levels

Sphingolipid (µM)	Serum	EDTA	Citrate	Heparin
SM	194.88 ± 16.55	193.39 ± 18.97	176.90 ± 15.36	192.56 ± 15.44
LacCer	12.004 ± 2.143	10.381 ± 1.595*	9.013 ± 1.934*	11.023 ± 1.067‡
HexCer	6.567 ± 0.966	6.481 ± 1.259	6.724 ± 1.148	8.477 ± 1.920*†‡
Cer	5.400 ± 1.343	5.670 ± 1.447	4.334 ± 0.966†	5.598 ± 1.480
Sphingosine	0.005 ± 0.001	0.006 ± 0.002	0.006 ± 0.001	0.008 ± 0.002*
dhSph	0.003 ± 0.001	0.004 ± 0.001	0.003 ± 0.001	0.004 ± 0.001
S1P	0.655 ± 0.154	0.305 ± 0.054*	0.242 ± 0.034*†	0.315 ± 0.042*‡
dhS1P	0.082 ± 0.018	0.044 ± 0.009*	0.033 ± 0.007*†	0.046 ± 0.010*‡
Cer1P	0.456 ± 0.087	0.539 ± 0.055	0.290 ± 0.056*†	0.262 ± 0.074*†
dhCer	0.332 ± 0.175	0.406 ± 0.228	0.268 ± 0.128	0.338 ± 0.160

Values are mean ± SD, n = 10 combined males and females under fasting conditions. * Anticoagulant statistically different from serum. ^† Anticoagulant statistically different from EDTA. ^‡ Heparin statistically different from citrate.