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. 2010 Oct;51(10):3074–3087. doi: 10.1194/jlr.D008532

TABLE 5.

Effect of freeze and thaw cycles on alterations of sphingolipid levels

Serum
Plasma EDTA
Sphingolipid T2 T3 T2 T3
C16-SM P = 0.0384 P = 0.0463
C16-Cer P = 0.0011
Sphingosine P = 0.0007
dhSph P = 0.0103 P = 0.0282
C18-Cer1P P = 0.0153
C18:1-Cer1P P = 0.0038
C26-Cer1P P = 0.0016
C16-LacCer P = 0.0434
C18:1-LacCer P = 0.0377 P = 0.0265
C16-HexCer P = 0.0322
C22:1-HexCer P = 0.0492
Hexosylsphingosine P = 0.0049

No P-value indicates absence of statistical difference from originally thawed sample (T0); no statistical differences in sphingolipid levels after T1.