Fig. 4.

ESI/MS analysis of PC and PE species in iPLA₂γ-WT and -KO skeletal muscles. Total lipids were extracted from thigh muscle homogenates and then subjected to ESI/MS analysis of PC (A and B) and PE (C and D). Representative ESI/MS profile (A and C) and quantitative results of four independent experiments. Means ± SE. ^*P < 0.05 versus control (n = 4) are shown in (B) and (D). Asterisks in (A) and (B) show altered molecular species. Abbreviations: IS, internal standard; PC, phosphatidylcholine; PE, phosphatidylethanolamine; iPLA₂, calcium-independent PLA₂; KO, knockout; SM, sphingomyelin; WT, wild-type.