TABLE 1.

Phospholipid compositions in skeletal muscle by TLC analysis

	Phospholipid Class
Genotype	CL	PE	PC	Phosphatidylglycerol
		Mean ± SEM (%)
WT	1.89 ± 0.28	12.57 ± 2.67	34.25 ± 8.9	5.33 ± 2.44
KO	0.96 ± 0.21a	11.08 ± 1.62	29.64 ± 4.71	1.75 ± 0.47

Total lipids were extracted from homogenates of thigh muscle from iPLA₂γ^+/+ (WT) and iPLA₂γ^−/− (KO) mice and separated on TLC silica gel plates. The TLC plates were stained by iodine vapor to visualize the phospholipids. After scraping the silica in bands of PC, PE, CL, and phosphatidylglycerol from the plates, the lipids were extracted from the silica two times with the method of Bligh and Dyer (25). Individual phospholipids were quantified by a molybdenum blue method. Quantitative data are means ± SE. CL, cardiolipin; PC, phosphatidylcholine; PE, phosphatidylethanolamine; iPLA₂, calcium-independent PLA₂; KO, knockout; WT, wild-type.

^aP < 0.05 versus WT (n = 6).