Fig. 3.

Circulating oxFA levels as predictors of NASH in patients with suspected NAFLD. A scoring system (oxNASH) that included 13-HODE/LA ratio, age, BMI, and AST showed best prediction of NASH diagnosis. (A) The AUC curve on the initial training cohort (n = 73) for oxNASH was estimated to be 0.83 (95% CI: 0.73, 0.93) and was found to be significantly higher than the AUCs of both that of serum AST 0.69 (95% CI: 0.56, 0.81) and serum ALT 0.56 (95% CI: 0.43, 0.70) (P < 0.01). (B) The box-whisker plot for oxNASH in the three groups of patients is represented with the lower boundary of the box indicating the 25th percentile, the line within the box indicating the median value, and the upper boundary of the box indicating the 75th percentile. The whiskers extend to the most extreme data point, which is no more than 1.5 times the interquartile range from the box. (C) The AUC in the independent validation group (n = 49) was estimated to be 0.74 (95% CI: 0.6, 0.88). Abbreviations: ALT, alanine transaminase; AST, aspartate transaminase; AUC, area under the curve; BMI, body mass index; HODE, hydroxy-octadecadenoic acid; NAFLD, nonalcoholic fatty liver disease; NASH, nonalcoholic steatohepatitis; oxFA, oxidized fatty acid.