Fig. 1.

Monocyte cytokine release promoted by LDL(+), LDL(–), and LDL(–) treated with HDL and apoAI. The release of MCP1, IL6, IL8, and IL10 expressed as ng/10⁶ cells was evaluated after incubation of monocytes with LDLs. LDL(–) treated with HDL and apoAI in coincubation conditions was named LDL(–)+HDL and LDL(–)+apoAI, respectively, and LDL(–)-HDL and LDL(–)-apoAI in preincubation. The X axis indicates the proportion of apoB:apoAI used in preincubation: 1 = 0.5 g apoB/L of LDL and 0.5 g apoAI/L of HDL. The final amount of LDL and HDL in each well was 150 μg of apoB (LDL) or apoAI (HDL). Release promoted by LDL(+) is shown in striped bars, by LDL(–) in gray bars, by LDL(–) in coincubation conditions in dotted bars, and in preincubation in white bars. Data are the mean ± SD of four experiments. Statistical differences with P = 0.068 are indicated as follows: ^* LDL(–) vs LDL(+); ^# LDL(–) treated versus LDL(–) untreated; ^$ preincubation versus coincubation, at the same lipoprotein concentration.