Fig. 2.

Elovl mRNA expressions in pachytene spermatocytes and round spermatids. RNA was obtained from round spermatid- and pachytene spermatocyte-enriched fractions. After reverse transcription using Oligo-dTs, the cDNA was amplified using specific primers for rat Elovl5 (407 bp predicted size), Elovl2 (568 bp predicted size) and β-actin (569 predicted size). 100 bp DNA ladder are shown on the left lane. Results are representative of at least three separate experiments giving similar results. Abbreviation: Elovl; fatty acid elongase.