TABLE 1.
Main components of cellular fractions
Pachythene Spermatocytes | Round Spermatids | Residual Bodies | |
---|---|---|---|
mg per 109 cells or particles | |||
Proteins | 199 ± 22a | 110 ± 17b | 25 ± 4c |
Phospholipids | 51 ± 3a | 21 ± 1b | 10 ± 0.5c |
Free cholesterol | 4.2 ± 0.1a | 1.9 ± 0.1b | 1.2 ± 0.1c |
Cholesterol esters | 0.15 ± 0.02a | 0.03 ± 0.01b | 0.02 ± 0.004b |
Ether-linked triglycerides | 0.41 ± 0.03a | 0.08 ± 0.003b | 0.03 ± 0.004c |
Diacylglycerols | 0.33 ± 0.02a | 0.20 ± 0.01b | 0.16 ± 0.01b |
Triacylglycerols | 2.46 ± 0.24a | 1.25 ± 0.11b | 1.56 ± 0.08c |
TAG/PL (mg/mg) | 0.05 ± 0.01a | 0.06 ± 0.01a | 0.16 ± 0.01b |
Chol/PL (mol/mol) | 0.18 ± 0.01a | 0.20 ± 0.01a | 0.25 ± 0.002b |
The amounts of protein, phospholipids, free cholesterol, and other neutral lipids (mg) are compared among pachytene spermatocytes, round spermatids, and residual bodies. The content of cholesterol esters, triacylglycerols, diacylglycerols, and ether-linked triglycerides was estimated from their fatty acids. ANOVA and Tukey's multiple comparison test was used for statistical analysis. Chol, cholesterol (free); PL, phospholipid; TAG, triacylglycerol. Row values with different superscript letters (a,b,c) point to significantly different values (P < 0.05).