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. 2010 Oct;51(10):2909–2921. doi: 10.1194/jlr.M006429

TABLE 1.

Main components of cellular fractions

Pachythene Spermatocytes Round Spermatids Residual Bodies
mg per 109 cells or particles
Proteins 199 ± 22a 110 ± 17b 25 ± 4c
Phospholipids 51 ± 3a 21 ± 1b 10 ± 0.5c
Free cholesterol 4.2 ± 0.1a 1.9 ± 0.1b 1.2 ± 0.1c
Cholesterol esters 0.15 ± 0.02a 0.03 ± 0.01b 0.02 ± 0.004b
Ether-linked triglycerides 0.41 ± 0.03a 0.08 ± 0.003b 0.03 ± 0.004c
Diacylglycerols 0.33 ± 0.02a 0.20 ± 0.01b 0.16 ± 0.01b
Triacylglycerols 2.46 ± 0.24a 1.25 ± 0.11b 1.56 ± 0.08c
TAG/PL (mg/mg) 0.05 ± 0.01a 0.06 ± 0.01a 0.16 ± 0.01b
Chol/PL (mol/mol) 0.18 ± 0.01a 0.20 ± 0.01a 0.25 ± 0.002b

The amounts of protein, phospholipids, free cholesterol, and other neutral lipids (mg) are compared among pachytene spermatocytes, round spermatids, and residual bodies. The content of cholesterol esters, triacylglycerols, diacylglycerols, and ether-linked triglycerides was estimated from their fatty acids. ANOVA and Tukey's multiple comparison test was used for statistical analysis. Chol, cholesterol (free); PL, phospholipid; TAG, triacylglycerol. Row values with different superscript letters (a,b,c) point to significantly different values (P < 0.05).