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. Author manuscript; available in PMC: 2010 Sep 10.
Published in final edited form as: Science. 2009 Sep 4;325(5945):1261–1265. doi: 10.1126/science.1173569

Fig. 3. P-REX2a and PI3K mutants cooperate.

Fig. 3

(A) P-REX2a expression levels in PTEN wild-type breast tumors without PIK3CA PI3K activating mutations (right) (p=0.014; two tailed t-test). (B) Proliferation in the absence of all growth factors and serum in MCF10A cells expressing P-REX2a and the PI3K mutant E545K (PI3K[EK]). (C) Microscopy of branched multilobulated mammospheres in MCF10A cells expressing P-REX2a or DHPH as indicated when grown in matrigel. Amount of pAKT shown (inset). All phase contrast images are displayed at 100x magnification. (D) Colony formation by MCF10A cells grown in soft agar. Cells expressing P-REX2a and PI3K[EK] as indicated (**p<0.01; t-test).