Fig. 2.

Relative apparent sizes of native and denatured ferritins. The ferritin and control (lacZ) proteins were expressed in U2-OS cells. a Denaturing SDS-PAGE western blot stained for L subunit. The length of the L*H and H*L polypeptide chain is approximately twice the size of L. b Native western blot stained for L. In their native conformation, the chimeric ferritins migrate in the same fashion as wild-type L. c Native western blot stained for the H subunit. The chimeric ferritins show the same molecular size as H but have more supramolecular species forming multimeric bands above the main band