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Effects of sorafenib (0.1–30 µM) on ERK-1/2 phosphorylation (2 h) in different human bladder carcinoma cell lines (RT4, T24, J82). Total and phosphorylated ERK-1/2 was detected by Western blotting. Original tracings and densitometrical analysis (means ± SEM) of five experiments are shown. *P < 0.05 versus control (anova /Bonferroni).
