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Effects of sorafenib (0.1–30 µM) on apoptosis of different human bladder carcinoma cell lines (RT4, T24, J82). Annexin V binding was measured by flow cytometry. PARP cleavage was assessed by Western blotting. Original FACS histograms and quantitative analysis of annexin V binding (left panel) and original Western blots with densitometrical analyses (right panel) are shown. Data are means ± SEM of five experiments. *P < 0.05 versus control (anova /Bonferroni).
