Table 1.
Viable cells % | Apoptotic cells % | Apoptotic-necrotic cells % | Necrotic cells % | |
---|---|---|---|---|
Blank | 95 ± 3 | 0 ± 2 | 0 ± 3 | 4 ± 1 |
LPS | 94 ± 5 | 5 ± 4 | 0 ± 1 | 1 ± 1 |
LPS + cucurbitacin R 100 µM | 40 ± 10 | 0 ± 12 | 51 ± 1 | 9 ± 3 |
LPS + cucurbitacin R 50 µM | 56 ± 8 | 41 ± 4 | 3 ± 2 | 3 ± 2 |
LPS + cucurbitacin R 25 µM | 69 ± 3 | 28 ± 3 | 1 ± 1 | 3 ± 2 |
LPS + cucurbitacin R 12.5 µM | 79 ± 10 | 19 ± 3 | 3 ± 2 | 1 ± 2 |
Cells were treated for 24 h with cucurbitacin R (12.5–100 µM) after LPS stimulation. Cells were collected and processed as described in Methods. Data are an average of three independent experiments.
LPS, lipopolysaccharide.