Table 2.
Microarray and Real-time PCR data of those genes which were differentially expressed to statistically significant degree
| Microarray Results | Realtime PCR Results | ||||
|---|---|---|---|---|---|
| Symbol | Accession # | Fold-change in USpA | Fold-change in AS | Fold-change in USpA | Fold-change in AS |
| OLR1 | NM_002543.2 | 33.7 * | 270.1 * | ||
| FOSB | NM_006732.1 | 26.2 *** | 10.8 ** | ||
| RGS1 | NM_002922.3 | 17.3 *** | 3.2 | 52.7 *** | 5.4 * |
| NR4A2 | NM_006186.2 | 17.0 *** | 3.5 | 49.7 *** | |
| CXCL2 | NM_002089.1 | 17.0 *** | 159.5 *** | 12.3 | |
| CCL20 | NM_004591.1 | 16.9 * | |||
| CCL3L3 | NM_001001437.2 | 14.8 *** | 13.1 * | ||
| ATF3 | NM_001030287.1 | 14.3 *** | 24.6 *** | ||
| CCL3L1 | NM_021006.4 | 13.2 ** | 14.4 * | ||
| HBEGF | NM_001945.1 | 12.8 *** | 2.8 | 27.4 *** | |
| IL-1B | NM_000576.2 | 11.6 *** | 6.9 * | ||
| IL-1A | NM_000575.3 | 11.4 * | 597.2 *** | ||
| CD83 | NM_004233.2 | 11.2 *** | 21.7 ** | ||
| NR4A3 | NM_173199.1 | 10.4 *** | 2.6 | ||
| OSM | NM_020530.3 | 10.3 *** | 4.5 * | ||
| PAI | NM_002575.1 | 9.8 *** | 33.5 *** | 3.5 | |
| IL8 | NM_000584.2 | 8.9 ** | 11.5 ** | ||
| SNF1LK | NM_173354.2 | 8.8 *** | 5.9 * | ||
| CCL3 | NM_002983.1 | 8.6 ** | 15.1 ** | ||
| NR4A1 | NM_173158.1 | 6.1 * | 8.1 | ||
| SOCS3 | NM_003955.3 | 4.7 *** | 2.7 | 3.4 * | |
| IL6 | NM_000600.1 | 4.5 | 35.4 *** | ||
| IL1RN | NM_173842.1 | 3.8 ** | |||
| DEFA1 | NM_004084.2 | -5.0 ** | -7.9 * | -6.9 | |
| CAMP | NM_004345.3 | -6.3 ** | -75.4 | ||
Cells with no numbers indicate that mean values are not different from control subjects
Fold-changes refer to comparison with healthy controls
p< 1 × 10 -5
p< 1 × 10 −4
p< 1 × 10 −2
Absence of asterisk next to numericals = p<0.05
All comparisons are to the healthy cohort.
OLR1 = oxidized low density lipoprotein receptor 1; FOSB = FBJ murine osteosarcoma viral oncogene homolog B; RGS1 = regulator of G-protein signaling 1; NR4A2 = nuclear receptor subfamily 4 group A member 2; CXCL2 = chemokine C-X-C motif ligand 2; CCL20 = chemokine C-C motif ligand 20; CCL3L3 = chemokine C-C motif ligand 3-like 3; ATF3 = activating transcription factor 3; CCL3L1 = chemokine C-C motif ligand 3-like 1; HBEGF = heparin-binding EGF-like growth factor; IL-1B = interleukin 1 beta; IL-1A = interleukin 1 alpha; NR4A3 = nuclear receptor subfamily 4 group A member 3; OSM = oncostatin M; PAI = placental plasminogen activator inhibitor; IL8 = interleukin 8; SNF1LK = salt-inducible kinase 1; CCL3 = chemokine C-C motif ligand 3; NR4A1 = nuclear receptor subfamily 4 group A member 1; SOCS3 = suppressor of cytokine signaling 3; IL6 = interleukin 6; IL1RN = interleukin 1 receptor antagonist; DEFA1 = defensin alpha 1; CAMP = cathelicidin antimicrobial peptide.
Cycling conditions for PCR assays: 95°C for 15 minutes, followed by amplification for 40 cycles of 94 °C for 15 seconds, 55 °C for 30 seconds and 72 °C for 30 seconds.