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Binding of SHALs to HLA-DR10-expressing and -nonexpressing live human cells. Enzyme-linked imunosorbent assay shows that tridentate, DvLPLLCtPCb-PLB, and dimeric (bis) tridentate, DvLPLLCtPCb-PPP)2LLB, SHALs bind to expressing cells, Raji and SuDHL4, but not to nonexpressing cells, CEM and Jurkat's. Experiments were conducted at RT (20°C) (upper) and, to inhibit endocytosis, at 4°C (lower). Cells were incubated with biotinylated SHALs, as described in Materials and Methods.
