Figure 5.
Confocal micrographs of washed live cells after incubation with chLym-1, tridentate (DvPLLCtPCbLB) or dimeric, tridentate ((DvPLLCtPCbPPP)2LLB) SHAL (left to right) in Raji (expressing; top row), and Jurkat's (nonexpressing; bottom row) cells. Lym-1 (left) showed Raji cell-surface membrane binding, whereas the tridentate (middle) and the dimeric, tridentate SHALs (right) showed intracellular Raji binding. There was no binding in Jurkat's cells. DAPI (blue) was used as the nuclear stain, and Alexa Fluor® (Invitrogen, Carlsbad, CA) (red) was used for the location of SHAL or Lym-1 in these merged laser images.