FIG. 2.

(A) IL13Rα2 consists of a large extracellular domain (ex), a transmembrane domain (TM), and a small intracellular domain (Int). The genetic sequence encoding only the extracellular domain of IL-13Rα2 was cloned into a bacterial expression vector containing an in-frame C-terminus his-tag (*), which was used to affinity purify IL13Rα2ex. (B) Coomassie stained sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blot analysis, using anti-IL13Rα2 anti-serum of purified recombinant IL13Rα2ex, used to immunize mice. (C) Schedule of IL13Rα2ex immunizations and tumor implantations in syngeneic immunocompetent groups of mice. (D) Post-tumor challenge survival of mice immunized with recombinant IL13Rα2ex protein. Mice were challenged with 5 × 10⁶ tumor cells at day 0. Mice were sacrificed when tumor volumes reached 400 mm³