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. 2010 Jun 26;49(10):1852–1861. doi: 10.1093/rheumatology/keq188

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© The Author(s) 2010. Published by Oxford University Press on behalf of The British Society for Rheumatology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4 — NGF expression in articular cartilage and bone. NGF-positive cells (brown) in vascular channels in the medial tibial plateau of patients with OA (A) and RA (C), but not in a non-arthritic control (B). (D) Chondrocytes displaying NGF immunoreactivity (brown) in superficial articular cartilage from a patient with OA (E and F). Co-localization within a vascular channel of NGF immunoreactivity (E, red) and a CGRP-immunoreactive nerve (F, black, arrow), demonstrated in serial tissue sections from a patient with OA. (A–D) DAB development with haematoxylin counterstain. (E) FastRed development. (F) Nickel-enhanced DAB development. Filled arrow heads: tidemark. Open arrow head: articular surface. Scale bar = 100 microns.