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Selection of the SSC with different doses of G418. (A) An SSC sphere in 0 μg/mL G418; after 1 day of G418 selection, few cells on the surface of the sphere appear to be degenerating and separating from the sphere in 150 and 200 μg/mL G418 groups (C,D). Cells appeared to be dying at 15 days selection in 150 and 200 μg/mL G418 groups (E,F). A few cells in the 50 μg/mL group were dying at 13 days of G418 selection (B).
