Table 2.
Transfection Efficiency and Survival of Porcine SSCs (24 h Posttransfection)
| Voltagea | Total cellsb(× 104) | Green cellsc(× 104) | % Green cellsd(% transfection) | % Survivale |
|---|---|---|---|---|
| 0 | 38.5 | 0 | 0 | 100 |
| 200 | 23.5 | 4 | 17.02 | 61.0 |
| 300 | 12 | 1 | 8.33 | 31.2 |
a
Single 1-ms pulse was administered in a 2-mm cuvette.
b
Number of total cells 24 h following transfection.
c
The total number of cells visually determined to express GFP.
d
The number of green cells divided by the total cells within a voltage group.
e
Survival was determined by dividing the number of total cells in the electroporated group by the number of total cells in the untransfected control.