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| Antibody | Raised in | Dilution |
| anti Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Cell signaling technologies, USA) | Rabbit | 1∶1000 |
| anti Erk1 (Zymed, CA, USA) | Mouse | 1∶500 |
| anti human β-actin (MP Biomedicals, OH, USA) | Mouse | 1∶2000 |
| anti-rabbit IgG (H + L) HRP conjugate (Jackson Immunoresearch Laboratories, PA, USA) | Donkey | 1∶4000 |
| anti-mouse IgG (H + L) HRP conjugate (Jackson Immunoresearch Laboratories, PA, USA) | Donkey | 1∶4000 |
