Figure 2. Enrichment of annotated RBPs in the protein microarray data.

The red line represents proteins annotated as RBPs, the blue line represents proteins not annotated as RBPs. Protein microarrays were probed with 500 nM polyA-selected RNA labeled with Cy5. Proteins were ranked by the ratio of normalized mean Cy5 signal to median microarray background. The Wilcoxon rank test was used to evaluate the significance of enrichment of known RBPs relative to proteins not annotated as RBPs.