Figure 11.

Signal improvement after AO compensation. (a) through (d) show histograms for the number of pixels according to their intensity; the x axis represents the intensity of pixels the same as in Fig. 9, except 100 (100 means 40 to 100% intensity pixels in the image). The y axis represents the number of pixels in the intensity range. The blue bars show the number of pixels before the compensation, and the red bars show the result after compensation. Each histogram was normalized to itself. (a) shows the distribution at 20 μm depth, (b) at 40 μm, (c) at 60 μm, and (d) at 80 μm imaging depth. (e) Percentage improvement according to imaging depth. The red line shows the improvement with background rejection (only the fluorescent area was calculated), and the blue line shows the improvement only with 90 to 100% intensity pixels. (Color online only.)